Currently, animal models of deep vein thrombosis (DVT) are mainly established by blocking venous return to induce blood stasis and thrombus formation, supplemented by injection of procoagulants and/or mechanical injury to vascular endothelial cells. Compared with modeling methods that directly damage veins such as electrical injury and mechanical injury, hypertonic saline injection causes less venous injury and better reflects the clinical pathogenesis of DVT.

- **Animals**: SD rats, male or female, 250–300 g
- **Model establishment**: The left femoral vein was incompletely ligated at the proximal end, and hypertonic saline was injected from the distal end of the left femoral vein. The femoral vein thrombosis model was successfully established 7 days later.
- **Positive drugs**: Cilostazol; Urokinase
- **Evaluation index**: H&E staining

**Vascular tissue H&E staining**