Alcoholism can be caused by long-term heavy drinking. It affects the nervous system, reproductive system, digestive system, and other systems, leading to functional damage in many organs and systems. The greater the average daily alcohol intake and the more years of drinking, the stronger the cumulative toxic effect of alcohol on the nervous system. Approximately 57.5% of patients with chronic alcoholism develop fatty liver, and 15% develop cirrhosis. This SOP aims to investigate the effects of chronic alcoholism in rats.

SD rats (male and female, 6–8 weeks old)
Modeling method: The model was successfully established by intragastric administration of 50% ethanol daily for 8 consecutive weeks.
Evaluation indicators: gross observation, body weight change measurement, HE staining

Gross observation of liver tissue

Measurement of body weight changes

HE staining of liver tissue