OVA-induced rat asthma model recapitulates many key features of allergic bronchial asthma, such as elevated specific IgE, edema, mucus hypersecretion, early- and late-phase asthmatic responses, increased and sustained eosinophilia and nonspecific AHR, eosinophilic infiltration, increased Th2 cytokines, and decreased interferon-γ (IFN-γ) secretion. The rat asthma model shares many similarities with human asthma: (1) Allergic responses are both IgE-mediated; (2) Biphasic responses consisting of immediate and late-phase reactions readily occur after challenge; (3) The onset time of the late-phase reaction is similar to that in asthmatic patients.

SD rats (both male and female, 6–8 weeks old)

Modeling method: Asthma model was established by intraperitoneal injection of OVA + aluminum adjuvant emulsion, followed by nebulization with 5% OVA in an inhalation chamber.

Evaluation indicators: Inflammatory cell count in bronchoalveolar lavage fluid (BALF), ELISA (IL-5 in BALF, serum IgE), HE staining and pathological scoring

Inflammatory cell count in bronchoalveolar lavage fluid (BALF)

ELISA (IL-5 in BALF, serum IgE)

HE staining and pathological scoring