Liver fibrosis is a dynamic healing response of the liver to chronic injury. Without effective treatment, it will eventually progress to cirrhosis. The main feature of liver fibrosis caused by chronic injury is abnormal deposition of extracellular matrix. In addition, various types of chronic injury can induce liver fibrosis, such as parasitic diseases, chronic viral infections (hepatitis B and C), immune disorders (autoimmune hepatitis), toxins, etc. Thioacetamide (TAA) is a hepatotoxin that causes severe cellular damage and exerts toxic effects on the synthesis of macromolecules (proteins and amino acids). TAA can induce liver injury, liver fibrosis and cirrhosis in experimental animals.

Wistar rats (male, 250–280 g)
Modeling method: Intraperitoneal injection of TAA (thioacetamide), twice a week for 3 consecutive weeks.
Evaluation indicators: HE staining, Masson staining, serum biochemical assay

HE staining of liver tissue

Masson staining of liver tissue

Serum biochemical assay