Osteoporosis (OP) is a metabolic bone disease characterized by decreased bone mass, deteriorated bone microarchitecture, increased bone fragility, and susceptibility to fractures. With aging, human bone mass follows a parabolic pattern, peaking at around 30 years old and gradually declining thereafter. The prevalence of osteoporosis in people over 60 reaches 60%–80%, and the incidence in women is three times higher than in men. A key contributing factor is the rapid drop in estrogen levels after menopause, which accelerates bone loss. This disease model of osteoporosis is established by surgically removing the bilateral ovaries of animals, thereby altering estrogen levels in vivo and inducing a sharp decline in estrogen.

SD rats (female, 3 months old)
Modeling method: Bilateral ovaries were ligated around the ovary and excised to establish an osteoporosis model.
Evaluation indicators: uterine index and ELISA detection, Micro‑CT, HE staining, TRAP staining

Uterine index and ELISA detection

Femur Micro‑CT

HE staining of the medial femoral condyle

TRAP staining of the medial femoral condyle