Joint instability and biomechanical disorders of weight-bearing joints are key pathogenic factors for osteoarthritis (OA). They induce chondrocyte degeneration, accelerate extracellular matrix degradation, and ultimately result in degenerative changes of articular cartilage. Surgical intervention or artificial trauma disrupts joint stability, alters mechanical stress on articular surfaces, and triggers intra-articular inflammatory responses, thereby successfully inducing OA lesions. This modeling method primarily mimics the pathological progression of post-traumatic osteoarthritis.

SD rats (male and female, 6–8 weeks old)
Modeling method: The anterior cruciate ligament was exposed and transected using micro-scissors. An osteoarthritis model was successfully established 8 weeks after surgery.
Evaluation indexes: HE staining, Safranin O-Fast Green staining

Bone tissue HE staining

Bone tissue Safranin O-Fast Green staining