This model is established based on doxorubicin, an anthracycline antitumor antibiotic with a quinone structure and potent cytotoxicity. Its core pathogenic mechanism is that doxorubicin intercalates into DNA to inhibit RNA synthesis, exerting direct nephrotoxicity. It damages DNA- and RNA-rich organelles in renal proximal tubular epithelial cells, such as nuclei and mitochondria, and eventually leads to focal segmental glomerulosclerosis in experimental animals. This modeling method features simple operation, stable pathological lesions and a high modeling success rate. It is well suitable for clinical research on drug efficacy evaluation and drug screening for minimal change disease.

SD rats (male, approximately 300 g)
Modeling method: Doxorubicin was injected via the tail vein twice at an interval of 7 days. A focal segmental glomerulosclerosis model was successfully established 40 days after the last injection.
Evaluation indexes: Urinary protein detection, PAS staining

Urinary protein detection

Renal tissue PAS staining