Primary membranous nephropathy is the leading pathological type of adult nephrotic syndrome, while the mechanisms underlying its tissue injury and disease progression remain incompletely clarified. The Heymann nephritis model is widely recognized as a classic animal model for investigating human membranous nephropathy. This model belongs to heterologous immune anti-glomerular basement membrane nephritis. The modeling mechanism is as follows: one species is immunized with renal cortical homogenate from another species to induce the production of anti-renal serum (anti-renal antibodies). The prepared anti-renal serum is then injected into healthy animals of the original donor species to trigger nephritis. Established by passive antibody administration, this lesion is termed Passive Heymann Nephritis (PHN). PHN serves as an ideal animal model for studying proteinuria and renal injury. Its advantages include exclusive renal targeting, low antibody dosage requirement, rapid lesion development, and pathological features highly analogous to human membranous nephropathy.

SD rats (male, 9–10 weeks old)
Modeling method: The prepared Fx1A rabbit antiserum was injected via the tail vein, and the Heymann nephritis model was successfully established after 10 days.
Evaluation indexes: HE staining, PAS staining

Renal tissue HE staining

Renal tissue PAS staining