This modeling method achieves chemical injury to the rat endometrium using 95% ethanol, mainly utilizing the dehydration effect of absolute ethanol on tissues and cells, as well as its ability to denature proteins. This modeling method is economical, has a short modeling time and stable model, and is quite suitable for establishing thin endometrium animal models used in the exploration of drug efficacy and related mechanisms.

SD rats (female, 8 weeks old)
Modeling method: 95% ethanol solution was perfused into the uterine horns, and the thin endometrium model was successfully established at the third estrous cycle.
Evaluation index: HE staining

Uterine HE staining