Ovalbumin (OVA) is an antigenic protein with a molecular weight of 45 000, possessing carrier activity and strong immunogenicity. Long-lasting antibodies can be produced after OVA sensitization, and this approach has been widely validated in domestic and international literature. Accordingly, OVA has become the most commonly used allergen for establishing allergic airway inflammation (AAI) animal models. The classic OVA sensitization protocol involves mixing freshly prepared OVA with an immunoadjuvant to enhance its immunogenicity, followed by repeated intraperitoneal injection for systemic sensitization. The method of constructing AAI animal models using OVA combined with aluminum hydroxide adjuvant is currently highly recognized and widely adopted by scholars worldwide.

Female BALB/c mice (6–8 weeks old)
Modeling method: The upper and lower allergic airway disease models were established by OVA nasal instillation, intraperitoneal injection and aerosol inhalation, respectively.
Evaluation index: HE staining

Nasal mucosa HE staining

Pulmonary tissue HE staining