Rats possess a strong endometrial repair capacity. Endometrial injury induced by a single intervention tends to be completely repaired within a short period. Moreover, animal models established by only one injury method cannot fully recapitulate the pathogenesis and contributing factors of intrauterine adhesions (IUA) in humans. Clinically, human IUA generally results from the combined effect of multiple damaging factors. Accordingly, establishing IUA animal models with two or more combined injury methods contributes to higher modeling success and better clinical relevance. Curettage is one of the major causes of pathological endometrial damage and IUA. Stable rat IUA models can be successfully constructed by combining curettage with 95% ethanol irritation.

Female SD rats, 6–8 weeks old
Modeling method: The uterine inner wall was scraped vigorously. Both ends of the injured segment were clamped, and absolute ethanol was injected into the local uterine cavity. The intrauterine adhesion model was successfully established 7 days later.
Evaluation indexes: uterine tissue scoring, HE staining

Uterine scoring

Uterine tissue HE staining