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Estradiol combined with mixed bacterial suspension-induced bacterial vaginitis model
Estradiol combined with mixed bacterial suspension-induced bacterial vaginitis model
Background
Bacterial vaginosis (BV) is a mixed infection mainly caused by Gardnerella vaginalis and multiple anaerobic bacteria, which disrupts the vaginal microecological balance. It is characterized by increased vaginal discharge, fishy-smelling leucorrhea, vulvar pruritus and burning sensation. It can be classified into haemophilic vaginitis, corynebacterial vaginitis, anaerobic vaginosis and gardnerella vaginitis.
In this study, exogenous estradiol injection induced hormonal imbalance in rats and damaged the vaginal mucosal barrier. Subsequent vaginal colonization with Escherichia coli and Staphylococcus aureus disrupted the normal vaginal flora via exogenous microbial infection, ultimately triggering infectious inflammatory lesions in the vagina.
Materials and methods
SD rats (female, 8 weeks old)
Modeling method: Benzoic acid estradiol was subcutaneously injected into the neck and back for 4 times. After the last injection, the vagina was mechanically scraped, followed by intravaginal perfusion of mixed bacterial suspension containing Staphylococcus aureus and Escherichia coli. The bacterial vaginitis model was successfully established 15 days later.
Evaluation indexes: gross observation, HE staining
Modeling method: Benzoic acid estradiol was subcutaneously injected into the neck and back for 4 times. After the last injection, the vagina was mechanically scraped, followed by intravaginal perfusion of mixed bacterial suspension containing Staphylococcus aureus and Escherichia coli. The bacterial vaginitis model was successfully established 15 days later.
Evaluation indexes: gross observation, HE staining
Test and verify
Gross observation
HE staining
