In this model, hyaluronidase is intravitreally injected to degrade hyaluronic acid in the vitreous scaffold and impair its water-binding capacity. The vitreous is then repeatedly aspirated with a syringe, and the narrow needle lumen disrupts the collagen fiber framework of the vitreous, leading to complete vitreous liquefaction within a short time. High-velocity fluid flow readily ruptures the retina and induces retinal breaks. The morphology of retinal breaks is closely related to the orientation of the impact needle. When the needle is perpendicular to the retina, crater-like or burst-shaped breaks are formed; low impact force results in round breaks; oblique needle operation produces round or oval retinal breaks. Local retinal detachment occurs simultaneously with break formation.

Japanese white rabbits (gender unlimited, 2–2.5 kg)
Modeling method: Hyaluronidase was intravitreally injected from the posterior limbus of the cornea. Repeated aspiration was performed after injection, and redundant vitreous fluid was extracted to successfully establish the retinal detachment model.
Evaluation indexes: gross observation, HE staining

Retinal HE staining