Dry eye disease is a disorder characterized by unstable tear film caused by insufficient tear secretion or excessive tear evaporation. At present, multiple approaches have been developed to establish animal models of dry eye, including environmental intervention, toxic agents, pharmacological administration, regulation of sex hormone levels, denervation of lacrimal glands and ocular surface nerves, induction of autoimmune responses in lacrimal glands, nutritional intervention, and surgical lacrimal gland resection. In this study, scopolamine was used to inhibit lacrimal gland secretion, so as to construct an animal model of insufficient tear production-type dry eye, providing a reference for basic research on dry eye disease.

BALB/c mice (female, 6–8 weeks old)
Modeling method: Scopolamine hydrobromide was alternately injected into both lower limbs of mice four times per day; benzalkonium chloride eye drops were administered topically three times daily. After continuous treatment for 10 consecutive days, the dry eye model was successfully established.
Evaluation indexes: corneal staining, tear secretion measurement, HE staining

Corneal staining

Tear secretion measurement