Obesity results from dysregulated energy metabolism in the body, and its exact pathogenesis has not been fully clarified. Current studies suggest that it is closely associated with genetics, central nervous system abnormalities, endocrine disorders, metabolic disturbances, nutritional imbalance and other factors, as well as environmental triggers. Nutritional obesity is a common type of obesity. At present, numerous studies have focused on diet-induced obesity animal models. Among them, high-fat diet-induced obese rat and mouse models are established by excessive caloric intake exceeding physiological metabolic demands. The surplus energy is converted and accumulated in the form of fat, ultimately leading to obesity.

C57BL/6 mice (male, 6–8 weeks old)
Modeling method: The obesity model was established by feeding with high-fat diet for 8 weeks.
Evaluation indexes: body weight measurement, HE staining

Body weight measurement

HE staining of epididymal adipocytes