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Porcine serum combined with D-galactosamine and lipopolysaccharide to induce acute-on-chronic liver failure model
Porcine serum combined with D-galactosamine and lipopolysaccharide to induce acute-on-chronic liver failure model
Background
Acute-on-chronic liver failure (ACLF) is a liver failure syndrome characterized by rapid deterioration in patients with relatively stable chronic liver disease under various acute injuries. In this protocol, intraperitoneal injection of porcine serum is used to induce chronic liver injury in rats. After the establishment of chronic liver disease, lipopolysaccharide combined with D-galactosamine is applied to induce acute injury in animals, leading to liver failure, so as to simulate the pathogenesis of human acute-on-chronic liver failure.
Materials and methods
Wistar rats (male, 120–150 g)
Modeling method: Porcine serum was injected twice a week for 11 consecutive weeks, followed by combined injection of D-galactosamine and lipopolysaccharide via tail vein to successfully establish an acute-on-chronic liver failure model.
Evaluation indicators: HE staining, Masson staining
Modeling method: Porcine serum was injected twice a week for 11 consecutive weeks, followed by combined injection of D-galactosamine and lipopolysaccharide via tail vein to successfully establish an acute-on-chronic liver failure model.
Evaluation indicators: HE staining, Masson staining
Test and verify
HE staining of liver tissue
Masson staining of liver tissue
