As an animal model of multiple sclerosis (MS), experimental autoimmune encephalomyelitis (EAE) provides convenient conditions for the study of the pathogenesis of MS. The incidence rate of animals in the experimental group using this method reaches 100%, and some animal models die due to severe illness. The reasons for the high incidence and severe condition of this model may be as follows: first, the large dosage of the antigen myelin basic protein (MBP). MBP is the main antigenic substance inducing EAE. Studies have shown that MBP can activate Th cells in the body, enabling them to cross the blood-brain barrier and attack MBP in the myelin sheath of their own nerves, thereby leading to demyelination of nerve cells and causing EAE or MS; second, the effect of newly cultured bacilli is better than that of finished vaccines. The toxins of bacilli can increase vascular permeability, making sensitized T cells easily cross the blood-brain barrier to attack the myelin sheath.

Lewis rats (female, 6–8 weeks old)
Modeling method: Experimental allergic encephalomyelitis model was induced by multi-point subcutaneous injection of MBP68-86 mixture.
Evaluation indicators: Clinical score, HE staining

Clinical neurological score

HE staining of hippocampal tissue