Modern studies have confirmed that animal models of traditional Chinese medicine blood-heat syndrome exhibit severe hyperviscosity, immune activation, as well as general manifestations such as skin purpura, fever, restlessness, red ears, congested eyes, reddish urine and dry stool. A blood-heat syndrome animal model is first established using heat-inducing traditional Chinese medicines with strong immunogenicity, followed by ovalbumin-induced skin allergy to finally establish an allergic purpura model.

Japanese white rabbits (male or female, 1.2–1.4 kg)
Modeling method: After 3 weeks of intragastric administration of traditional Chinese medicine water decoction, sensitization was performed via intraperitoneal injection of ovalbumin emulsion, and the Henoch-Schönlein purpura model was finally established by challenging with ovalbumin via marginal ear vein injection.
Evaluation indicators: ELISA assay, HE staining

ELISA assay

HE staining of renal tissue

HE staining of skin tissue