In diabetic patients, microvascular and peripheral neuropathy can lead to cutaneous nutritional disorders, resulting in skin injury, secondary infection, and other conditions. Once infected, the wound repair capacity is extremely poor, often causing intractable ulcerative wounds. Chronic skin ulcers are common surgical diseases, among which diabetic skin ulcers represent one type, characterized by repeated episodes and persistent non-healing. In diabetic patients, skin infection can exacerbate diabetic symptoms and is difficult to control. Without timely treatment, the lesion may progress deeply, even leading to amputation. In this study, a type 2 diabetes mellitus model was first established, followed by creating a dorsal wound to construct a diabetic wound model.

SD rats (male, 5–6 weeks old)

Modeling method: A type 2 diabetes mellitus model was established by high-fat diet combined with intraperitoneal injection of STZ. On this basis, a wound was created on the back to construct a diabetic wound model.

Positive drug: Jing Wan Hong Ointment

Evaluation indicators: ELISA assay, HE staining, Masson staining, TUNEL staining

ELISA assay of skin wound tissue

HE staining of skin wound tissue

Masson staining of skin wound tissue

TUNEL staining of skin wound tissue