This model simulates the process of human evolution from quadrupedal locomotion to bipedal walking, and is internationally recognized as a classic experimental animal model with considerable advantages. This modeling method has two characteristics. First, it induces rats to maintain an upright posture to mimic human bipedal walking, altering the mechanical loading on the lumbar spine, which more closely resembles the pathogenesis of lumbar disc herniation caused by intervertebral disc degeneration following human upright posture. Second, the bilateral forelimb amputation method for inducing upright posture in rats is a classic technique, and the resulting lumbar intervertebral disc degeneration more closely approximates the pathogenesis of human lumbar disc herniation.

SD rats (male, 3 weeks old)

Modeling method: The forelimbs were separated from the scapulae by dissection starting at the joint capsule below the shoulder joint to establish the lumbar intervertebral disc degeneration model.

Evaluation indicators: pain threshold measurement, HE staining, toluidine blue staining

Pain threshold measurement (mechanical pain, thermal pain)

HE staining of lumbar intervertebral disc

Toluidine blue staining of lumbar intervertebral disc