Necrotizing enterocolitis (NEC) is a leading cause of mortality from gastrointestinal diseases in premature infants. With advances in neonatal care, the survival rate of very low birth weight and extremely low birth weight infants has continued to improve, yet the incidence and associated mortality of NEC have not declined. According to incomplete statistics, among premature infants with a birth weight below 1500 g, the incidence of NEC is approximately 10%, with a mortality rate ranging from 20% to 30%, which rises with increasing disease severity. Neonatal mouse necrotizing enterocolitis is an intestinal necrotic disease caused by intestinal infection in neonatal mice due to various factors. It represents a severe acute abdominal disorder of the digestive tract in neonatal mice, characterized by typical manifestations including vomiting, abdominal distension, and hematochezia.

Newborn C57BL/6 mice (3–5 days old)
Modeling method: The pups were briefly placed in a hypoxic chamber and then immediately transferred to a 4 °C refrigerator (rapid cold stimulation after hypoxia), twice daily. The necrotizing enterocolitis model was successfully established after 4 days.
Evaluation indicators: gross observation, body weight measurement, HE staining

Gross observation of the colon

Body weight measurement

HE staining of colonic tissue