Autoimmune thyroiditis (AIT) has an incidence second only to hyperthyroidism, accounting for approximately 20% of thyroid diseases. Current methods for establishing AIT animal models mainly include T-cell depletion, immune-mediated induction, and high-iodine induction. AIT induced by Ts cell depletion via Tx-X treatment offers the advantage of not requiring exogenous administration of autoantigens or adjuvants. However, immunizing animals with thyroid autoantigen components such as thyroglobulin, thyroid peroxidase, or thyroid-stimulating hormone receptor, alone or combined with adjuvants (e.g., LPS, CFA), remains a well-recognized classic method.

Wistar rats (female, approximately 200 g)
Modeling method: Rats were housed with deionized water containing NaI until the end of modeling. Primary immunization: subcutaneous injection of thyroglobulin (Tg) combined with complete Freund’s adjuvant twice; booster immunization: subcutaneous injection of thyroglobulin with incomplete Freund’s adjuvant. The autoimmune thyroiditis model was successfully established 7 days after the last injection.
Evaluation indicators: serum ELISA, HE staining

Serum ELISA

HE staining of thyroid tissue