Intra-articular injection of sodium iodoacetate (MIA) in animals is a well-recognized and effective method for inducing osteoarthritis (OA). Sodium iodoacetate is an inhibitor of glyceraldehyde-3-phosphate dehydrogenase activity, which can induce chondrocyte death and thereby cause cartilage damage. Its loss of proteoglycan matrix and functional joint damage are similar to those observed in human OA. Advantages of this model: the degenerative changes observed in tissues are comparable to those of the ACLT method. In addition to acute inflammation, the model also exhibits pain approximately 10 days after injection, making it the most commonly used OA model for testing the analgesic effects of related drugs. This minimally invasive model features high reproducibility and ease of operation.

SD rats (male, 7–8 weeks old)
Modeling method: The osteoarthritis model was successfully established 7 days after intra-articular injection of sodium iodoacetate into the knee joint.
Evaluation indicators: X-ray examination, HE staining, Safranin O-Fast Green staining

Knee joint X-ray film

HE staining of knee joint

Safranin O-Fast Green staining of knee joint