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Mouse ear hypertrophic scar model established by circular ear corneal punch incision
Mouse ear hypertrophic scar model established by circular ear corneal punch incision
Background
Human deep skin wounds heal through fibrous repair. During this process, granulation tissue proliferates at the wound base, supporting epidermal proliferation at the wound margin and migration toward the wound center, which eventually covers the defect and completes healing. Due to the limited repair capacity of the skin, large-area skin defects often require a long healing time. Meanwhile, certain underlying diseases may interfere with the wound healing process, leading to refractory wounds, for which there are still no satisfactory clinical solutions.
Although fibrous repair restores skin integrity and the epidermal barrier function, it mainly relies on granulation tissue proliferation to fill the skin defect, inevitably resulting in scar formation after healing. Ordinary scars formed after skin wound healing quickly enter the maturation phase, characterized by cellular apoptosis, collagen remodeling, decreased vascular density, gradual fading of scar color, and softening of texture.
Excessive inflammatory responses or mechanical tension on the wound during skin wound healing may induce hypertrophic scars. Hypertrophic scars exhibit a prominent proliferative phase with active cell proliferation, massive collagen deposition, and abnormally high expression of various growth factors (such as transforming growth factor-β1, insulin-like growth factor 1, etc.). These changes lead to elevated, erythematous, and rigid scars prone to contracture, which impair local appearance and function, cause severe psychological distress, and remain a major clinical challenge.
Materials and methods
ICR mice (female, 6–8 weeks old)
Modeling method: A circular incision was made on the ventral surface of the mouse ear using a corneal punch, and the tissue within the incision was removed. The mouse ear hypertrophic scar model was successfully established 14 days later.
Evaluation indicators: gross observation, HE staining, Masson staining, Vancouver Scar Scale
Modeling method: A circular incision was made on the ventral surface of the mouse ear using a corneal punch, and the tissue within the incision was removed. The mouse ear hypertrophic scar model was successfully established 14 days later.
Evaluation indicators: gross observation, HE staining, Masson staining, Vancouver Scar Scale
Test and verify
Ear gross observation
HE staining of skin tissue
Masson staining of skin tissue
Vancouver Scar Scale
