Currently, the preparation of deep vein thrombosis (DVT) animal models primarily involves obstructing venous return to induce blood stasis and thrombosis formation, supplemented by the injection of procoagulant substances or (and) mechanical injury to vascular endothelial cells. Compared to modeling methods that directly damage veins, such as electrical injury or mechanical injury techniques, the injection of hypertonic saline causes less venous damage and better aligns with the pathophysiologic

SD rats (sex not specified, 250–300 g)
Model creation method: The proximal end of the left femoral vein was incompletely ligated. Hypertonic saline was injected into the distal end of the left femoral vein. After 7 days, a femoral vein thrombosis model was successfully established.
Positive Drug 1: Cilostazol; Positive Drug 2: Urokinase
Evaluation Criteria: Hematoxylin and Eosin (HE) Staining

血管HE染色